MSU Center for Water Sciences
Quantitative polymerase chain reaction (qPCR) methods can provide faster results than the current culture-based method for monitoring beaches. However, qPCR methods are relatively new- EPA released new criteria based on qPCR in 2012- and require specific equipment and expertise. Because implementing a new method can be challenging, we have developed guidance to help those new to qPCR through the process. Check out the site to learn more- you can jump straight to a section by clicking one of the below links:
This manual aims to provide technical assistance to laboratories that will be using qPCR for the first time. The content describes the steps necessary to decide whether qPCR is feasible, to outfit a qPCR laboratory, to collect and process samples, to apply quantitative polymerase chain reaction method for the detection of enterococci in recreational waters (Method 1611), and to interpret and report results.
The manual and individual chapters are pdf files. You will need Adobe Reader to view the files- click here to get Adobe Reader if you don't already have it.
Download entire manual:
A. Aslan, J. Kinzelman, E. Dreelin, T. Anan'eva, and J. Lavender
Download individual chapters:
Updated manual for E. coli Method C!
We have updated the manual for E. coli testing using EPA Method C. Click here for a pdf of the entire updated manual.
See qPCR in action. This video will lead you through the qPCR analysis step-by-step.
Dr. Asli Aslan provided the technical expertise to develop this training video. Dr. Amol Pavangadkar produced and directed the video with his team in Communication Arts & Sciences.
Methods and Criteria
EPA Method 1611 (pdf)
No organized training sessions currently scheduled.
Are you a Michigan lab setting up qPCR? MSU offers training sessions at MSU and visits to your lab. Contact Dr. Tiong Gim Aw to schedule a session.
SimpleScience Introduction to PCR video
The goal of MIqPCR listserv is to provide a forum for those in Michigan using qPCR for monitoring water. To be added to the listserv, contact Dr. Erin Dreelin.
Still have questions? We can help! Contact Dr. Tiong Gim Aw with questions about the qPCR method or setting up a qPCR lab.
This project is currently funded by the Michigan Department of Environmental Quality. Production of the manual and training video was funded by an EPA Great Lakes Restoration Initiative grant to the Michigan Department of Environmental Quality.